Research Group Microfluid Biotechnique

Nachwuchsgruppenleiterin / Head of Research Group

Dr. rer. nat. habil. Jialan Cao-Riehmer

Visiting address:
Prof. Schmidt Str. 26

Heliosbau Room 1103
98693 Ilmenau

Mail address:
Postfach 10 05 65
98684 Ilmenau

E-Mail: Jialan.Cao@tu-ilmenau.de

Forschung / Research focus

The Microfluid Biotechnique Research Group is focused on the development of microfluid devices, sensors and optimization of microfluid screening processes, cultivation and characterization of

Soil Microorganisms,
Photosynthesis-active microorganisms (cyanobacterium, algae) and
Plant cell cultures (microspores, protoplasts)

J. Cao-Riehmer

Team

M. Sc. Paulina Marczakiewicz-Perera (PhD Student)

Paulina Marczakiewicz-Perera

+49 3677 69-3689

Forschungsgebiet: Anwendung der Mikrofluidtechnik für die Pflanzenbiotechnologie

Research focus: Application of microfluid technology for plant cells: The goal of her research is to optimize a microfluid platform for cultivation of various plant cell cultures, such as protoplasts and somatic cells. Subsequently, she investigate effect of external factors (growth regulators, salts content, light etc.) on the early divisions at nearly single cell level.

M. Sc. Ting Xie (PhD student)

Ting Xie

+49 3677 69-3715

Forschungsgebiet: Entwicklung eines mikrofluidischen Systems für die Selektion und Anwendung bioelektrochemisch aktiver Bodenbakterien für die CO₂-Fixierung

Research focus: Development of a microfluidic system for the selection and application of bioelectrochemically active microorganisms for CO₂ fixation

Alumi

Frau Bayan Nasr

Masterarbeit: Dosis-Wirkungsscreening von Pseudomonas Putida KT2440 gegen leichte und schwere Lanthanide und Entwicklung eines Protokolls für die Segment-RNAseq-basierte Genexpressionsanalyse

Frau Nadia Davina Prasetija

Masterarbeit: Untersuchung des Wachstums und der Sensitivität von Mikroalgen und Cyanobakterien gegen Effektoren unter verschiedenen Lichtkonditionen in mikrofluidischen Photobioreaktoren (µPBR)

Herr Felix Richter

Masterarbeit: Einsatz der Mikrofluidsegmenttechnik zur Untersuchung der Pflanzenembryogenese am Beispiel von Brassica napus

Frau Nadhrah Musfira

Bachelorarbeit: Characterization of Microfluidic Devices for the Generation of multidimensional Concentration Space and for the Investigation of Growth of Cyanobacteria

Our paper and artwork has been selected as cover for Journal Lab on a Chip

Induction of embryogenic development in haploid microspore stem cells in droplet-based microfluidics

In a recently published study, together with University Freiburg, TU Ilmenau and Company ScreenSys, we did studies of droplet-based microfluidics for application in plant cells. Here, we present our droplet-based tube system suitable for implementation in an automated, miniaturized system with a microchannel as the central transport and processing unit to achieve induction of embryogenic development in haploid stem cells.

Link: xlink.rsc.org

http://xlink.rsc.org/?DOI=D2LC00788F

Publikationen / publication

N. Prasetija, S. Schneider, T. Xie, J. Cao*, „Droplet-Based Microfluidic Photobioreactor (µ-PBR) as a Growth Optimization Tool for Cyanobacteria and Microalgae” Environments 2024, 11(11), 255; doi.org/10.3390/environments11110255
J. M. Köhler*, L. Ehrhardt, P. M. Günther, J. Cao, „Soil Bacteria in Archaeology: What Could Rank Abundance Functions Report on Ancient Human Impacts on Microbial Communities?” Microorganisms 2024, 12, 2243. doi.org/10.3390/microorganisms12112243
L. Gorniak, S. Bucka, B. Nasr, J. Cao, S. Hellmann, T. Schäfer, M. Westermann, J. Bechwar, C.-E. Wegner*, „Changes in growth, lanthanide uptake, and gene expression in Pseudomonas alloputida KT2440 in response to light and heavy lanthanides” mSphere 2024 doi:doi.org/10.1101/2024.04.15.589537
J. M. Köhler*, L. Ehrhardt, J. Cao, „Mikrobiologisches Gedächtnis: NGS-Untersuchungen in der Archäologie”, BioSpektrum 2024, 05: 515-519, DOI: 10.1007/s12268-024-2285-8
J. M. Köhler*, L. Ehrhardt, P. M. Günther, M. Böhme, J. Cao „Low Abundant Bacteria Reflect Soil Specificity – Analysis of Bacterial Communities from Archaeological Investigation of Pre-Industrial Saline Ash Deposits of Bad Dürrenberg (Germany)“ environments 2024, 11(3), 42 https://doi.org/10.3390/environments11030042
T. Xie, L. Ehrhardt, P.M. Günther, J.M. Köhler and J. Cao*„Current to Biomass: Media Optimization and Strain Selection from Cathode-Associated Microbial Communities in a Two-Chamber Electro-Cultivation Reactor” environments 2023, 10,97.https://doi.org/10.3390/environments10060097
J.M. Köhler, L. Ehrhardt, J. Cao, F. Möller, T. Schüler and P.M. Günther “Beta-Diversity Enhancement by Archaeological Structures: Bacterial Communities of an Historical Tannery Area of the City of Jena (Germany) Reflect the Ancient Human Impact” ecologies 2023, 4(2) 325-343 https://doi.org/10.3390/ecologies4020021
T. Xie, J.M. Köhler, S. Heyder, P.M. Günther and J. Cao* „Microfluidically-assisted Isolation and Characterization of Achromobacter spanius from Soils for Microbial Degradation of Synthetic Polymers and Organic Solvents” environments 2022, 9(12), 147 https://doi.org/10.3390/environments9120147
J. Cao*, U. Pliquett, L. Yang, S. Wiedermeier, B. Cahill and J.M. Köhler “Combination of Impedance and Optical Measurements in Serial Sensing for Microfluidic Dose-Response Investigations of Soil Microorganisms” 2022 Sensors and Actuators B, https://doi.org/10.1016/j.snb.2022.132688
L. Ehrhardt, P. M. Günther, M. Böhme, M. Köhler and J. Cao “Three Soil Bacterial Communities from an Archaeological Excavation Site of an Ancient Coal Mine near Bennstedt (Germany) Characterized by 16S r-RNA Sequencing” Environments 2022, 9, 115 https://doi.org/10.3390/environments9090115
T. Henkel, G. Mayer, J. Hampl, J. Cao, L Ehrhardt, A. Schober, G. A. Groß* “From Microtiter Plates to Droplets - There and Back Again” Micromachines 2022, 13, 1022 https://doi.org/10.3390/mi13071022
J. Cao, C. Chande and J. M. Köhler “Microtoxicology by Microfluidic Instrumentation: a review” lab Chip 2022, 22, 2600-2623 https://doi.org/10.1039/D2LC00268J
F. Richter, M. Chen, P. Schaub, F. Wüst, D. Zhang, S. Schneider, G. A. Groß, P. Mäder, O. Dovzhenko, K. Palme, J. M. Köhler und J. Cao* „Induction of embryogenic development in haploid microspore stem cells in droplet-based microfluidics” Lab Chip 2022, https://doi.org/10.1039/D2LC00788F
J. Cao*, D. Russo, T. Xie, G.A. Groß and J. Zedler* „Droplet-based Microfluidic for Media Optimization Studies of Cyanobacteria" Sci. Report 2022,12:15536 https://doi.org/10.1038/s41598-022-19773-6
J.M. Köhler, N.Beetz, P. M. Günther,F. Möller and J. Cao,” Extremophiles in Soil Communities of Former Copper Mining Sites of the East Harz Region (Germany) Reflected by Re‐Analyzed 16S rRNA Data”, Microorganisms 2021, 9, 7 https://doi.org/10.3390/microorganisms9071422
J. Cao*, C. Chande, F. Kalensee, T. Schüler, M Köhler, „ Microfluidically supported characterization of responses of R. erythropolis strains isolated from different soils on Cu-, Ni-, and Co-Stress” Brazilian Jour. of Microbiol 2021,52, 1405-1415 https://doi.org/10.1007/s42770-021-00495-2
P. Schumann; F. Kalensee; J. Cao; A. Criscuolo; D. Clermont; J. M. Köhler; J. P. Meier-Kolthoff; M. Neumann-Schaal; B. J. Tindall; R. Pukall, “Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb. nov., of Haloactinobacterium album as Ruania alba comb. nov” Int. Jour. Of Sys. Evo. Microbiogy 2021, 71, 4 https://doi.org/10.1099/ijsem.0.004769
J. M. Köhler, N. Beetz, P. M. Günther, F. Möller, T. Schüler, J. Cao, “Microbial community types and signature-like soil bacterial patterns from fortified prehistoric hills of Thuringia (Germany)” Community Ecology 2020, 21, 107-120 https://doi.org/10.1007/s42974-020-00017-4
J. Cao, F. Richter, M. Kastl. et al. „Droplet-based screening for the investigation of microbial nonlinear dose-response characteristics system, background, and examples” Micromachines 2020, 6, 557, https://doi.org/10.3390/mi11060577
M. Kielpinski, O. Walter, J. Cao, T. Henkel, M. Köhler, A. Groß, “Microfluidic chamber design for controlled droplet expansion and coalescence”, Micromachines 2020, 11, 4, 394 https://doi.org/10.3390/mi11040394
J. Cao, F. Kalensee, P. M. Günther, J. M. Köhler „Microsegmented flow-assisted miniaturized culturing for isolation and characterization of heavy metal-tolerant bacteria” Int J Environ Sci Te, 2020, 17 https://doi:10.1007/s13762-019-02424-1
J. M. Köhler, F. Kalensee, J. Cao and P. M. Günther, “Hadesarchaea and other extremophile bacteria from ancient mining areas of the East Harz region (Germany) suggest an ecological longterm memory of soil”, SN App. Sci, 2019, 1: 839 https://DOI:10.1007/s42452-019-0874-9
Köhler, J. M., Kalensee, F., Günther, P. M., Schüler, T. & Cao, J. “The Local Ecological Memory of Soil: Majority and Minority Components of Bacterial Communities in Prehistorical Urns from Schöps (Germany)”. Int. J. of Env. Res. 2018, 12, 575-584, https://doi:10.1007/s41742-018-0116-9
J. Cao, L. Hafermann, J. M. Köhler. „Mikrofluidik – großes Potenzial in kleinen Tropfen”, BioSpektrum 2017, 23 (4): 394-397, DOI:10.1007/s12268-017-0816-2
J. Cao, T. Schüler, J. M. Köhler. „Stochastically reduced communities—Microfluidic compartments as model and investigation tool for soil microorganism growth in structure ed spaces”, Journal Eng. Life Science, 2017, 17, 792-800, https://DOI:10.1002/elsc.201600264
J. Cao, J. M. Köhler, “Droplet-based Microfluidics for Microtoxicological Studie”, Review, Journal Eng. Life Science, 2015, 15(3), 306-317, https://DOI:10.1002/elsc.201400074
J. Cao, S. Nagl, E. Kothe and J.M. Köhler, “Oxygen sensor microparticles for monitoring bacterial growth and characterization of dose/response functions in microfluidic screenings”, Microchim Acta, 2015, 182, 385-394, https://DOI:10.1007/s00604-014-1341-3
J. Cao, R. Schultheiß, S. Schneider, A. Schober, J. M. Köhler and G. A. Groß “'DROP-BY-DROP' - TOOLS FOR DROPLET-BASED MICRO-FLUIDIC PROCESSING”, Microsyst Technol., 2015, 21, 539-548, https://DOI:10.1007/s00542-013-1981-y
K. Wetzel, J. Cao, E. Kothe and J. M. Köhler, “Changing growth behavior of heavy-metal tolerant bacteria: Media optimization using droplet-based microfluidics”, Journal Eng. Life Science, 2015, 15(3), 327-332, https://DOI:10.1007/s00253-013-5147-8
J. Cao, J. Goldhan, K. Martin and J.M. Köhler, “Investigation of mixture toxicity of widely used drugs caffeine and ampicillin in the presence of an ACE inhibitor on bacterial growth using droplet-based microfluidic technique”, Green Processing and Synthesis. 2013, 2(6), 591–601, https://DOI:10.1515/gps-2013-0078
J. Cao, D. Kürsten, K. Krause, E. Kothe, K. Martin, M. Roth and J.M. Köhler, “Application of Micro-Segmented Flow for Two-dimensional Characterization of the Combinatorial Effect of Zinc and Copper Ions on Metal-tolerant Streptomyces Strains”, Appl. Microbiol. Biotechnol. 2013, 97, 8923-8930, https://DOI:10.1007/s00253-013-5147-8
J. Cao, D. Kürsten, S. Steffen, A. Knauer, P.M. Günther, J.M. Köhler, “Uncovering toxicological complexity by multi-dimensional screenings in micro segmented flow: Modulation of antibiotic interference by nano particles”, Lab Chip, 2012, 12 (3), 474-484, https://DOI:10.1039/c1lc20584f
J. Cao, D. Kürsten, S. Schneider, and J. M. Köhler, "Stimulation and Inhibition of Bacterial Growth by Caffeine Dependent on Chloramphenicol and a Phenolic Uncoupler - A Ternary Toxicity Study Using a Microfluid Segment Technique", J. Biomed. Nanotechnol. 2012, 8 (5), 770-778, https://DOI:10.1166/jbn.2012.1447
D. Kürsten, J. Cao, A. Funfak, P. Müller, J. M. Köhler, "Cultivation of Chlorella vulgaris in micro fluid segments and micro toxicological determination of their sensitivity against CuCl2 in the nanoliter range", Engineering in Life Science, 2011, 11(6), 580-587, https://DOI:10.1002/elsc.201100023
A. Funfak, J. Cao, A. Knauer, K. Martin, J. M. Köhler, "Synergistic effects of metal nanoparticles and a phenolic uncoupler using microdroplet-based two-dimensional approach", Journal of Environmental Monitoring, 2011, 13(2), 410-415 https://DOI:10.1039/C0EM00162G

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Nachwuchsgruppenleiterin / Head of Research Group

Forschung / Research focus

Team

M. Sc. Paulina Marczakiewicz-Perera (PhD Student)

M. Sc. Ting Xie (PhD student)

Alumi

Our paper and artwork has been selected as cover for Journal Lab on a Chip

Publikationen / publication

Studienangebot

Uni erleben

Studienstart

Studierendenleben

Quicklinks

Studienangebot

Studienorganisation

Studieren ist mehr

Information und Beratung

Quicklinks

Berufseinstieg

Gründungsservice

Weiterbildung

Alumni

Quicklinks

Nachwuchsgruppenleiterin / Head of Research Group

Forschung / Research focus

Team

M. Sc. Paulina Marczakiewicz-Perera (PhD Student)

M. Sc. Ting Xie (PhD student)

Alumi

Our paper and artwork has been selected as cover for Journal Lab on a Chip

Publikationen / publication

Publications