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Horak, Iryna; Prylutska, Svitlana; Krysiuk, Iryna; Luhovskyi, Serhii; Hrabovsky, Oleksii; Tverdokhleb, Nina; Franskevych, Daria; Rumiantsev, Dmytro; Senenko, Anton; Evstigneev, Maxim; Drobot, Liudmyla; Matyshevska, Olga; Ritter, Uwe; Piosik, Jacek; Prylutskyy, Yuriy;
Nanocomplex of Berberine with C60 fullerene is a potent suppressor of Lewis lung carcinoma cells invasion in vitro and metastatic activity in vivo. - In: Materials, ISSN 1996-1944, Bd. 14 (2021), 20, 6114, insges. 15 S.
Im Titel ist "60" tiefgestellt

Effective targeting of metastasis is considered the main problem in cancer therapy. The development of herbal alkaloid Berberine (Ber)-based anticancer drugs is limited due to Ber low effective concentration, poor membrane permeability, and short plasma half-life. To overcome these limitations, we used Ber noncovalently bound to C60 fullerene (C60). The complexation between C60 and Ber molecules was evidenced with computer simulation. The aim of the present study was to estimate the effect of the free Ber and C60-Ber nanocomplex in a low Ber equivalent concentration on Lewis lung carcinoma cells (LLC) invasion potential, expression of epithelial-to-mesenchymal transition (EMT) markers in vitro, and the ability of cancer cells to form distant lung metastases in vivo in a mice model of LLC. It was shown that in contrast to free Ber its nanocomplex with C60 demonstrated significantly higher efficiency to suppress invasion potential, to downregulate the level of EMT-inducing transcription factors SNAI1, ZEB1, and TWIST1, to unblock expression of epithelial marker E-cadherin, and to repress cancer stem cells-like markers. More importantly, a relatively low dose of C60-Ber nanocomplex was able to suppress lung metastasis in vivo. These findings indicated that omplexation of natural alkaloid Ber with C60 can be used as an additional therapeutic strategy against aggressive lung cancer.



https://doi.org/10.3390/ma14206114
Ovsiienko, Iryna V.; Tsaregradskaya, Tatiana L.; Shpylka, D. O.; Matzui, Lyudmila Yu.; Saenko, Galina V.; Ritter, Uwe; Len, Tatiana A.; Prylutskyy, Yuriy I.;
Magnetoresistance of carbon nanotubes filled by iron. - In: IEEE Xplore digital library, ISSN 2473-2001, (2021), S. NMM05-1-NMM05-5

Paper presents the results of experimental investigations of magnetoresistance of filled with iron multi-walled carbon nanotubes. Multi-walled carbon nanotubes have been prepared by pyrolysis of benzene in a tubular quartz furnace at a temperature of 950˚C with use ferrocene as a source of iron. The obtained by this method carbon nanotubes contain in the inner cavity the particles of the magnetic phase, namely iron, iron carbide and iron oxides in various concentrations. The electrical resistance of bulk specimens of modified carbon nanotubes have been carried out in the temperature interval from 4.2 K to 293 K and in magnetic field up to 2 T. It is shown that magnetoresistance of modified carbon nanotubes is determined by a combination of two effects: the giant magnetoresistance effect and anisotropic magnetoresistance effect, moreover, the relative contribution of each effect depends on the concentration of the magnetic phase.



https://doi.org/10.1109/NAP51885.2021.9568395
Alam, Shahidul; Nádaždy, Vojtech; Váry, Tomáš; Friebe, Christian; Meitzner, Rico; Ahner, Johannes; Anand, Aman; Karuthedath, Safakath; Castro, Catherine S. P. De; Göhler, Clemens; Dietz, Stefanie; Cann, Jonathan; Kästner, Christian; Konkin, Alexander; Beenken, Wichard J. D.; Anton, Arthur Markus; Ulbricht, Christoph; Sperlich, Andreas; Hager, Martin; Ritter, Uwe; Kremer, Friedrich; Brüggemann, Oliver; Schubert, Ulrich Sigmar; Ayuk Mbi Egbe, Daniel; Welch, Gregory C.; Dyakonov, Vladimir; Deibel, Carsten; Laquai, Frédéric; Hoppe, Harald;
Uphill and downhill charge generation from charge transfer to charge separated states in organic solar cells. - In: Journal of materials chemistry, ISSN 2050-7534, Bd. 9 (2021), 40, S. 14463-14489

It is common knowledge that molecular energy level offsets of a type II heterojunction formed at the donor-acceptor interface are considered to be the driving force for photoinduced charge transfer in organic solar cells. Usually, these offsets - present between molecular energy levels of the donor and acceptor - are obtained via cyclic voltammetry (CV) measurements of organic semiconductors cast in a film or dissolved in solution. Simply transferring such determined energy levels from solution or film of single materials to blend films may be obviously limited and not be possible in full generality. Herein, we report various cases of material combinations in which novel non-fullerene acceptors did not yield successful charge transfer, although energy levels obtained by CV on constituting single materials indicate a type II heterojunction. Whilst the integer charge transfer (ICT) model provides one explanation for a relative rise of molecular energy levels of acceptors, further details and other cases have not been studied so far in great detail. By applying energy-resolved electrochemical impedance spectroscopy (ER-EIS) on several donor-acceptor combinations, a Fano-like resonance feature associated with a distinctive molecular energy level of the acceptor as well as various relative molecular energy level shifts of different kinds could be observed. By analyzing ER-EIS and absorption spectra, not only the exciton binding energy within single materials could be determined, but also the commonly unknown binding energy of the CT state with regard to the joint density of states (jDOS) of the effective semiconductor. The latter is defined by transitions between the highest occupied molecular orbitals (HOMO) of the donor and the lowest unoccupied molecular orbitals (LUMO) of the acceptor. Using this technique among others, we identified cases in which charge generation may occur either via uphill or by downhill processes between the charge transfer exciton and the electronic gap of the effective semiconductor. Exceptionally high CT-exciton binding energies and thus low charge generation yields were obtained for a case in which the donor and acceptor yielded a too intimate blend morphology, indicating π-π stacking as a potential cause for unfavorable molecular energy level alignment.



https://doi.org/10.1039/D1TC02351A
Grebinyk, Anna; Prylutska, Svitlana; Grebinyk, Sergii; Evstigneev, Maxim; Krysiuk, Iryna; Skaterna, Tetiana; Horak, Iryna; Sun, Yanfang; Drobot, Liudmyla; Matyshevska, Olga; Prylutskyy, Yuriy; Ritter, Uwe; Frohme, Marcus;
Antitumor efficiency of the natural alkaloid berberine complexed with C60 fullerene in Lewis lung carcinoma in vitro and in vivo. - In: Cancer nanotechnology, ISSN 1868-6966, Bd. 12 (2021), 24, insges. 18 S.

Berberine (Ber) is a herbal alkaloid with pharmacological activity in general and a high anticancer potency in particular. However, due to its low bioavailability, the difficulty in reaching a target and choosing the right dose, there is a need to improve approaches of Ber use in anticancer therapy. In this study, Ber, noncovalently bound to a carbon nanostructure C60 fullerene (C60) at various molar ratios of the components, was explored against Lewis lung carcinoma (LLC).



https://doi.org/10.1186/s12645-021-00096-6
Hurmach, Vasyl V.; Platonov, Maksim O.; Prylutska, Svitlana V.; Scharff, Peter; Prylutskyy, Yuriy I.; Ritter, Uwe;
C60 fullerene against SARS-CoV-2 coronavirus: an in silico insight. - In: Scientific reports, ISSN 2045-2322, Bd. 11 (2021), 17748, insges. 12 S.
Im Titel ist "60" tiefgestellt

Based on WHO reports the new SARS-CoV-2 coronavirus is currently widespread all over the world. So far > 162 million cases have been confirmed, including > 3 million deaths. Because of the pandemic still spreading across the globe the accomplishment of computational methods to find new potential mechanisms of virus inhibitions is necessary. According to the fact that C60 fullerene (a sphere-shaped molecule consisting of carbon) has shown inhibitory activity against various protein targets, here the analysis of the potential binding mechanism between SARS-CoV-2 proteins 3CLpro and RdRp with C60 fullerene was done; it has resulted in one and two possible binding mechanisms, respectively. In the case of 3CLpro, C60 fullerene interacts in the catalytic binding pocket. And for RdRp in the first model C60 fullerene blocks RNA synthesis pore and in the second one it prevents binding with Nsp8 co-factor (without this complex formation, RdRp can't perform its initial functions). Then the molecular dynamics simulation confirmed the stability of created complexes. The obtained results might be a basis for other computational studies of 3CLPro and RdRp potential inhibition ways as well as the potential usage of C60 fullerene in the fight against COVID-19 disease.



https://doi.org/10.1038/s41598-021-97268-6
Strutynska, Nataliia; Malyshenko, Anna; Tverdokhleb, Nina; Evstigneev, Maxim; Vovchenko, Ludmila; Prylutskyy, Yuriy; Slobodyanik, Nikolai; Ritter, Uwe;
Design, characterization and mechanical properties of new Na+, CO32--apatite/alginate/C60 fullerene hybrid biocomposites. - In: Journal of the Korean Ceramic Society, ISSN 2234-0491, Bd. 58 (2021), 4, S. 422-429
Im Titel sind "+" und "2-" hochgestellt, "3" und "60" tiefgestellt

Nanoparticles (20-50 nm) of Na+, CO32--containing calcium phosphate (Na: 1.49 wt% and C: 1.53 wt%) with apatite-type structure were prepared by precipitation method from aqueous solution. According to FTIR spectroscopy data, the partial substitution of phosphate by carbonate (B-type) realized in the apatite-type structure. Obtained Na+, CO32--hydroxyapatite (HAP) was used for the preparation of hybrid biocomposites with Alginate (Alg) with weight ratio HAP: Alg = 1:1 or 2:1 and C60 fullerene (C60; from 0.2 to 4 wt%) and their mechanical properties were determined. It was found, that sample with weight ratio HAP: Alg = 2:1 and containing 4.0 wt% of C60 has the highest Young's modulus 429 MPa comparing with other determined samples. The structure modeling of the investigated system showed that the formation of triple complexes Na+, CO32--HAP-Alg-C60 is stabilized by solvophobic and stacking interactions. The created biocomposites can be used as an effective implant material for bone restoration.



https://doi.org/10.1007/s43207-020-00107-z
Chernykh, Mariia; Zavalny, Dmytro; Sokolova, Viktoriya; Ponomarenko, Stanislav; Prylutska, Svitlana; Kuziv, Yuliia; Chumachenko, Vasyl; Marynin, Andrii; Kutsevol, Nataliya; Epple, Matthias; Ritter, Uwe; Piosik, Jacek; Prylutskyy, Yuriy;
A new water-soluble thermosensitive star-like copolymer as a promising carrier of the chemotherapeutic drug doxorubicin. - In: Materials, ISSN 1996-1944, Bd. 14 (2021), 13, 3517, insges. 13 S.

A new water-soluble thermosensitive star-like copolymer, dextran-graft-poly-N-iso-propilacrylamide (D-g-PNIPAM), was created and characterized by various techniques (size-exclusion chromatography, differential scanning calorimetry, Fourier-transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) spectroscopy). The viability of cancer cell lines (human transformed cervix epithelial cells, HeLa) as a model for cancer cells was studied using MTT and Live/Dead assays after incubation with a D-g-PNIPAM copolymer as a carrier for the drug doxorubicin (Dox) as well as a D-g-PNIPAM + Dox mixture as a function of the concentration. FTIR spectroscopy clearly indicated the complex formation of Dox with the D-g-PNIPAM copolymer. The size distribution of particles in Hanks solution was determined by the DLS technique at different temperatures. The in vitro uptake of the studied D-g-PNIPAM + Dox nanoparticles into cancer cells was demonstrated by confocal laser scanning microscopy. It was found that D-g-PNIPAM + Dox nanoparticles in contrast to Dox alone showed higher toxicity toward cancer cells. All of the aforementioned facts indicate a possibility of further preclinical studies of the water-soluble D-g-PNIPAM particles behavior in animal tumor models in vivo as promising carriers of anticancer agents.



https://doi.org/10.3390/ma14133517
Kriničnij, Viktor I.; Yudanova, Evgeniya I.; Denisov, Nikolay N.; Konkin, Aleksei A.; Ritter, Uwe; Bogatyrenko, Victor R.; Konkin, Alexander L.;
Light-induced electron paramagnetic resonance study of charge transport in fullerene and nonfullerene PBDB-T-based solar cells. - In: The journal of physical chemistry, ISSN 1932-7455, Bd. 125 (2021), 22, S. 12224-12240

https://doi.org/10.1021/acs.jpcc.1c03427
Nozdrenko, Dmytro; Matvienko, Tetiana; Vygovska, Oksana; Bogutska, Kateryna; Motuziuk, Olexandr; Nurishchenko, Natalia; Prylutskyy, Yuriy; Scharff, Peter; Ritter, Uwe;
Protective effect of water-soluble C60 fullerene nanoparticles on the ischemia-reperfusion injury of the muscle soleus in rats. - In: International journal of molecular sciences, ISSN 1422-0067, Bd. 22 (2021), 13, 6812, insges. 13 S.
Im Titel ist "60" tiefgestellt

The biomechanical parameters of muscle soleus contraction in rats and their blood biochemical indicators after the intramuscular administration of water-soluble C60 fullerene at doses of 0.5, 1, and 2 mg/kg 1 h before the onset of muscle ischemia were investigated. In particular, changes in the contraction force of the ischemic muscle soleus, the integrated power of the muscle, the time to achieve the maximum force response, the dynamics of fatigue processes, and the parameters of the transition from dentate to smooth tetanus, levels of creatinine, creatine kinase, lactate and lactate dehydrogenase, and parameters of prooxidant-antioxidant balance (thiobarbituric acid reactive substances, hydrogen peroxide, and reduced glutathione and catalase) were analyzed. The positive therapeutic changes in the studied biomechanical and biochemical markers were revealed, which indicate the possibility of using water-soluble C60 fullerenes as effective prophylactic nanoagents to reduce the severity of pathological conditions of the muscular system caused by ischemic damage to skeletal muscles.



https://doi.org/10.3390/ijms22136812
Nozdrenko, Dmytro; Abramchuk, Olga; Prylutska, Svitlana; Vygovska, Oksana; Soroca, Vasil; Bogutska, Kateryna; Khrapatyi, Sergii; Prylutskyy, Yuriy; Scharff, Peter; Ritter, Uwe;
Analysis of biomechanical parameters of muscle soleus contraction and blood biochemical parameters in rat with chronic glyphosate intoxication and therapeutic use of C60 fullerene. - In: International journal of molecular sciences, ISSN 1422-0067, Bd. 22 (2021), 9, 4977, insges. 11 S.
Im Titel ist "60" tiefgestellt

https://doi.org/10.3390/ijms22094977