Modeling of single-walled carbon nanotube binding to nitric oxide synthase and guanylate cyclase molecular structures. - In: Neurophysiology, ISSN 1573-9007, Bd. 52 (2020), 2, S. 110-115
Previously, we have demonstrated that water dispersible single-walled carbon nanotubes (SWCNTs) may be used in low therapeutic doses in antihypertensive therapy as promising agents capable of activating constitutive nitric oxide synthase (NOS) in spontaneously hypertensive rats, thus increasing the NO production in central and peripheral elements of the cardiovascular system [1]. Here we confirm this effect by docking and molecular dynamics simulations, clearly showing that SWCNTs may interact with NOS and guanylate cyclase molecular structures.
https://doi.org/10.1007/s11062-020-09859-0
Systemic administrations of water-dispersible single-walled carbon nanotubes: activation of NOS in spontaneously hypertensive rats. - In: Neurophysiology, ISSN 1573-9007, Bd. 52 (2020), 2, S. 101-109
Priority data have been obtained on the effects of repeated systemic administrations of water-dispersible single-walled carbon nanotubes (SWCNTs) to spontaneously hypertensive rats with respect to constitutive NO-synthase (cNOS). As is known, NO is an inhibitory transmitter in the cardiovascular system. It was found that the systemic (i.p., subcutaneous, and i.m.) introductions of SWCNTs during two weeks resulted in considerable elevations of the NO2- level (a marker of NO bioavailability) in the blood of experimental hypertensive animals. Thus, SWCNTs may be used in the future for antihypertensive therapy as a novel agent capable of activating cNOS and, thus, increasing the NO production in central and peripheral elements of the cardiovascular system.
https://doi.org/10.1007/s11062-020-09858-1
C60 fullerene governs doxorubicin effect on metabolic profile of rat microglial cells in vitro. - In: Molecular pharmaceutics, ISSN 1543-8392, Bd. 17 (2020), 9, S. 3622-3632
Im Titel ist "60" tiefgestellt
Background: C60 fullerenes and their derivatives are actively investigated for the use in neuroscience. Applications of these nanoscale materials require the examination of their interaction with different neural cells, especially with microglia, because these cells, like other tissue resident phagocytes, are the earliest and most sensitive responders to nanoparticles. The aim of this study was to investigate the effect of C60 fullerene and its nanocomplex with doxorubicin (Dox) on the metabolic profile of brain-resident phagocytes - microglia - in vitro. Methods: Resting microglial cells from adult male Wistar rats were used in experiments. Potential C60 fullerene targets in microglial cells were studied by computer simulation. Microglia oxidative metabolism and phagocytic activity were examined by flow cytometry. Griess reaction and arginase activity colorimetric assay were used to explore arginine metabolism. Results: C60 fullerene when used alone did not influence microglia oxidative metabolism and phagocytic activity but shifted arginine metabolism toward the decrease of NO generation. Complexation of C60 fullerene with Dox (C60-Dox) potentiated the ability of the latter to stimulate NO generation. Conclusion: The capability of C60 fullerenes used alone to cause anti-inflammatory shift of microglia arginine metabolism makes them a promising agent for the correction of neuroinflammatory processes involved in neurodegeneration. The potentiating action of C60 fullerene on the immunomodulatory effect of Dox allows us to consider the C60 molecule as an attractive vehicle for this antitumor agent.
https://doi.org/10.1021/acs.molpharmaceut.0c00691
Evaluation of the biocompatibility of water-soluble pristine 60 fullerenes in rabbit. - In: BioNanoScience, ISSN 2191-1649, Bd. 10 (2020), 3, S. 721-730
Im Titel ist "60" tiefgestellt
C60 fullerenes have proved their therapeutic effects and efficacy by the results of countless experiments. For further usage of these nanoparticles, the systematic toxicological investigations are required. Blood compatibility should be studied for C60 fullerenes due to the potential blood contact. Currently, available data is not systematic and has not provided insights into possible side effects of C60 fullerenes on blood components. In this study, water-soluble pristine C60 fullerenes were tested in vitro to assess their biocompatibility in rabbit. The blood compatibility has been evaluated looking at the impact of C60 fullerenes on erythrocyte integrity, platelet aggregation, and some blood factors involved in coagulation. Our results revealed that C60 fullerenes cannot elicit hemolysis at studied concentrations and did not show any effect on coagulation process. C60 fullerenes in concentration-dependent manner increased ADP-dependent platelet aggregation and changed the key kinetic parameters of these processes. C60 fullerenes inhibited thrombin amidolytic activity but did not affect the activities of other studied coagulation factors. The prothrombotic property of C60 fullerenes could be the potential risk factor that leads to enhancement of vascular thrombosis. The ability of fullerene to inhibit thrombin activity is important for the pharmacological use of these carbon nanoparticles as anticoagulant agents.
https://doi.org/10.1007/s12668-020-00762-w
New nanostructured apatite-type (Na+,Zn2+,CO32-)-doped calcium phosphates : Preparation, mechanical properties and antibacterial activity. - In: Journal of molecular structure, ISSN 0022-2860, Bd. 1222 (2020), 128932
Im Titel sind "+", "2+" und "2-" hochgestellt, "3" tiefgestellt
Nanoparticles with the sizes in the range (20-30) nm of apatite-type Na+,Zn2+,CO32--doped calcium phosphates were prepared from aqueous solution of Na+-Ca2+-Zn2+-NO3--CO32--PO43- system at molar ratios Ca2+/PO43- =1.67, CО32-/РО43- = 1, Zn2+:Ca2+ = 1:100 or 2:100. The elemental analysis showed growth of Zn2+ content in composition of samples from 0.61 to 1.95 wt% at increasing of Zn2+ amount in an initial solution. According to FTIR and Raman data, B-type substitution of PO43- by CO32- realized in apatite-type calcium phosphates. The mechanical properties study for prepared phases showed the dependence of Young's modulus and compressive strength on Zn2+ amount in their composition. Growth of S. epidermidis after the contact with synthesized apatite containing Na+ (0.25 wt%), Zn2+ (0.61 wt%) and CO32- (1.18 wt%) was significantly delayed with an extension of lag time from 1 to 13-14 h. The prepared sample can be considered as a new prospective biomaterial with antibacterial potential.
https://doi.org/10.1016/j.molstruc.2020.128932
Water-soluble pristine C60 fullerene inhibits liver alterations associated with hepatocellular carcinoma in rat. - In: Pharmaceutics, ISSN 1999-4923, Bd. 12 (2020), 9, 794, S. 1-20
Richtiger Name des Verfassers: Eric Täuscher
https://doi.org/10.3390/pharmaceutics12090794
Microbial community types and signature-like soil bacterial patterns from fortified prehistoric hills of Thuringia (Germany). - In: Community ecology, ISSN 1588-2756, Bd. 21 (2020), 2, S. 107-120
16S rRNA profiling has been applied for the investigation of bacterial communities of surface soil samples from forest-covered areas of ten prehistorical ramparts from different parts of Thuringia. Besides the majority bacterial types that are present in all samples, there could be identified bacteria that are highly abundant in some places and absent or low abundant in others. These differences are mainly related to the acidity of substrate and distinguish the communities of lime stone hills from soils of sand/quartzite and basalt hills. Minority components of bacterial communities show partially large differences that cannot be explained by the pH of the soil or incidental effects, only. They reflect certain relations between the communities of different places and could be regarded as a kind of signature-like patterns. Such relations had also been found in a comparison of the data from ramparts with formerly studied 16S rRNA profiling from an iron-age burial field. The observations are supporting the idea that a part of the components of bacterial communities from soil samples reflect their ecological history and can be understood as the "ecological memory" of a place. Probably such memory effects can date back to prehistoric times and might assist in future interpretations of archaeological findings on the prehistoric use of a place, on the one hand. On the other hand, the genetic profiling of soils of prehistoric places contributes to the evaluation of anthropogenic effects on the development of local soil bacterial diversity.
https://doi.org/10.1007/s42974-020-00017-4
Biomimetic reconstruction of the hematopoietic stem cell niche for in vitro amplification of human hematopoietic stem cells. - In: PLOS ONE, ISSN 1932-6203, Bd. 15 (2020), 6, e0234638, insges. 17 S.
https://doi.org/10.1371/journal.pone.0234638
P-204 C60 fullerene inhibits hepatocellular carcinoma development and metastasis: in vitro and in vivo studies. - In: Annals of oncology, ISSN 1569-8041, Bd. 31 (2020), S. S156-147
https://doi.org/10.1016/j.annonc.2020.04.286
Identification and evaluation of cell- growth-inhibiting bDtBPP-analogue degradation products from phosphite antioxidants used in polyolefin bioprocessing materials. - In: Analytical and bioanalytical chemistry, ISSN 1618-2650, Bd. 412 (2020), 19, S. 4505-4518
https://doi.org/10.1007/s00216-020-02736-z