Publikationen am Institut für Chemie und Biotechnik

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Bartsch, Heike; Weise, Frank; Gomez, Houari Cobas; Gongora-Rubio, Mario Ricardo;
Cost-effective sensor for flow monitoring in biologic microreactors. - In: IEEE sensors journal. - New York, NY : IEEE, ISSN 1558-1748, Bd. 21 (2021), 19, S. 21314-21321
Grebinyk, Anna; Prylutska, Svitlana; Grebinyk, Sergii; Evstigneev, Maxim; Krysiuk, Iryna; Skaterna, Tetiana; Horak, Iryna; Sun, Yanfang; Drobot, Liudmyla; Matyshevska, Olga; Prylutskyy, Yuriy; Ritter, Uwe; Frohme, Marcus;
Antitumor efficiency of the natural alkaloid berberine complexed with C60 fullerene in Lewis lung carcinoma in vitro and in vivo. - In: Cancer nanotechnology : basic, translational and clinical research.. - Wien [u.a.] : Springer, ISSN 1868-6966, Bd. 12 (2021), S. 1-18

Berberine (Ber) is a herbal alkaloid with pharmacological activity in general and a high anticancer potency in particular. However, due to its low bioavailability, the difficulty in reaching a target and choosing the right dose, there is a need to improve approaches of Ber use in anticancer therapy. In this study, Ber, noncovalently bound to a carbon nanostructure C60 fullerene (C60) at various molar ratios of the components, was explored against Lewis lung carcinoma (LLC).
Hurmach, Vasyl V.; Platonov, Maksim O.; Prylutska, Svitlana V.; Scharff, Peter; Prylutskyy, Yuriy I.; Ritter, Uwe;
C60 fullerene against SARS-CoV-2 coronavirus: an in silico insight. - In: Scientific reports. - [London] : Macmillan Publishers Limited, part of Springer Nature, ISSN 2045-2322, Bd. 11 (2021), S. 1-12
- Im Titel ist "60" tiefgestellt

Based on WHO reports the new SARS-CoV-2 coronavirus is currently widespread all over the world. So far > 162 million cases have been confirmed, including > 3 million deaths. Because of the pandemic still spreading across the globe the accomplishment of computational methods to find new potential mechanisms of virus inhibitions is necessary. According to the fact that C60 fullerene (a sphere-shaped molecule consisting of carbon) has shown inhibitory activity against various protein targets, here the analysis of the potential binding mechanism between SARS-CoV-2 proteins 3CLpro and RdRp with C60 fullerene was done; it has resulted in one and two possible binding mechanisms, respectively. In the case of 3CLpro, C60 fullerene interacts in the catalytic binding pocket. And for RdRp in the first model C60 fullerene blocks RNA synthesis pore and in the second one it prevents binding with Nsp8 co-factor (without this complex formation, RdRp can't perform its initial functions). Then the molecular dynamics simulation confirmed the stability of created complexes. The obtained results might be a basis for other computational studies of 3CLPro and RdRp potential inhibition ways as well as the potential usage of C60 fullerene in the fight against COVID-19 disease.
Kronfeld, Klaus-Peter; Ellinger, Thomas; Köhler, Michael;
Micro flow photochemical synthesis of Ca-sensitive fluorescent sensor particles. - In: Engineering in life sciences. - Weinheim : Wiley-VCH, ISSN 1618-2863, Bd. 21 (2021), 8/9, S. 518-526

Fluorescence probes have widely been used for detecting and imaging Ca2+-enriched parts of cells but more rarely for quantitative determination of concentrations. In this study we show how this can be achieved by a novel approach using hydrogel particles. In a microfluidic co-flow arrangement spherical droplets were generated from an aqueous solution of acrylamide, N,N'-methylenebisacrylamide crosslinker and photoinitiator and subsequently photo-cured in situ yielding gel particles in a sub millimeter range. These particles were separated, dried under reduced pressure and re-swollen in water containing Rhod-5N tri potassium salt as calcium ion selective fluorescence probe. After that the particles were dried again and stored for further investigations. Upon exposure of dried particles to calcium chloride solutions they swell and take up Ca2+-ions forming a strong fluorescing complex with Rhod-5N. Thus, fluorescence intensity increases with calcium ion concentration. Up to ca. 0.50 mM the enhancement effect is strong and then becomes considerably weaker. The intensity-concentration-dependence is well described by an equation derived from the equilibrium of the formation of a 1:1 Ca2+:Rhod-5N complex. The particles allow for a fast optical determination of Ca2+-concentrations up to 0.50 mM in analyte volumes down to below 10 [my]L.
Köhler, Michael; Kluitmann, Jonas; Günther, Mike;
Metal nanoparticles as free-floating electrodes. - In: Encyclopedia. - Basel : MDPI, ISSN 2673-8392, Bd. 1 (2021), 3, S. 551-565

Colloidal metal nanoparticles in an electrolyte environment are not only electrically charged but also electrochemically active objects. They have the typical character of metal electrodes with ongoing charge transfer processes on the metal/liquid interface. This picture is valid for the equilibrium state and also during the formation, growth, aggregation or dissolution of nanoparticles. This behavior can be understood in analogy to macroscopic mixed-electrode systems with a free-floating potential, which is determined by the competition between anodic and cathodic partial processes. In contrast to macroscopic electrodes, the small size of nanoparticles is responsible for significant effects of low numbers of elementary charges and for self-polarization effects as they are known from molecular systems, for example. The electrical properties of nanoparticles can be estimated by basic electrochemical equations. Reconsidering these fundamentals, the assembly behavior, the formation of nonspherical assemblies of nanoparticles and the growth and the corrosion behavior of metal nanoparticles, as well as the formation of core/shell particles, branched structures and particle networks, can be understood. The consequences of electrochemical behavior, charging and self-polarization for particle growth, shape formation and particle/particle interaction are discussed.
Köhler, Michael;
Challenges for nanotechnology. - In: Encyclopedia. - Basel : MDPI, ISSN 2673-8392, Bd. 1 (2021), 3, S. 618-631

The term "Nanotechnology" describes a large field of scientific and technical activities dealing with objects and technical components with small dimensions. Typically, bodies that are in-at least-two dimensions smaller than 0.1 [my]m are regarded as "nanobjects". By this definition, a lot of advanced materials, as well as the advanced electronic devices, are objects of nanotechnology. In addition, many aspects of molecular biotechnology as well as macromolecular and supermolecular chemistry and nanoparticle techniques are summarized under "nanotechnology". Despite this size-oriented definition, nanotechnology is dealing with physics and chemistry as well as with the realization of technical functions in the area between very small bodies and single particles and molecules. This includes the shift from classical physics into the quantum world of small molecules and low numbers or single elementary particles. Besides the already established fields of nanotechnology, there is a big expectation about technical progress and solution to essential economic, medical, and ecological problems by means of nanotechnology. Nanotechnology can only meet these expectations if fundamental progress behind the recent state of the art can be achieved. Therefore, very important challenges for nanotechnology are discussed here.
Zhou, Xue-Quan; Mytiliniou, Maria; Hilgendorf, Jonathan; Zeng, Ye; Papadopoulou, Panagiota; Shao, Yang; Dominguez, Maximilian Paradiz; Zhang, Liyan; Hesselberth, Marcel B. S.; Bos, Erik; Siegler, Maxime A.; Buda, Francesco; Brouwer, Albert M.; Kros, Alexander; Koning, Roman I.; Heinrich, Doris; Bonnet, Sylvestre;
Intracellular dynamic assembly of deep-red emitting supramolecular nanostructures based on the Pt Pt metallophilic interaction. - In: Advanced materials. - Weinheim : Wiley-VCH, ISSN 1521-4095, Bd. 33 (2021), 37, S. 1-13
Zeußel, Lisa; Mai, Patrick; Sharma, Sanjay; Schober, Andreas; Ren, Shizhan; Singh, Sukhdeep;
Colorimetric method for instant detection of lysine and arginine using novel Meldrum's acid-furfural conjugate. - In: ChemistrySelect. - Weinheim : Wiley-VCH, ISSN 2365-6549, Bd. 6 (2021), 27, S. 6834-6840

In the past few years Meldrum's acid furfural conjugate (MAFC) have been extensively explored as starting material for the synthesis of photo switchable donor acceptor stenhouse adducts (DASA). Hereby, we have explored the interaction of MAFC with various amino acids. To our surprise, nitrogen rich amino acids like lysine and arginine interact spontaneously with MAFC to give colored adduct immediately, whereas other amino acids, including nitrogen rich histidine, didn't show any coloration. Naked eye detection of lysine in benign solvent make this reagent an attractive new entry to the collection of chemosensors for the colorimetric detection of lysine and arginine. Intense coloration corresponds to the absorption at 514 nm under UV-Vis spectrometer. Lowest concentration of 100 m can be detected with UV-Vis spectrometer. NMR titrations reveals that the appearance of color is due to ring opening of a furfural that leads to the formation of conjugated triene species. Compared to previously reported chemosensors for lysine and arginine, MAFC offers advantages including simple synthesis, easy handling, high speed, low cost, good sensitivity/selectivity.
Cao, Jialan; Chande, Charmi; Kalensee, Franziska; Schüler, Tim; Köhler, Michael;
Microfluidically supported characterization of responses of Rhodococcus erythropolis strains isolated from different soils on Cu-, Ni-, and Co-stress. - In: Brazilian journal of microbiology : publication of the Sociedade Brasileira de Microbiologia.. - [Cham] : Springer International Publishing, ISSN 1678-4405, Bd. 52 (2021), 3, S. 1405-1415

We present a new methodological approach for the assessment of the susceptibility of Rhodococcus erythropolis strains from specific sampling sites in response to increasing heavy metal concentration (Cu2+, Ni2+, and Co2+) using the droplet-based microfluid technique. All isolates belong to the species R. erythropolis identified by Sanger sequencing of the 16S rRNA. The tiny step-wise variation of metal concentrations from zero to the lower mM range in 500 nL droplets not only provided accurate data for critical metal ion concentrations but also resulted in a detailed visualization of the concentration-dependent response of bacterial growth and autofluorescence activity. As a result, some of the isolates showed similar characteristics in heavy metal tolerance against Cu2+, Ni2+, and Co2+. However, significantly different heavy metal tolerances were found for other strains. Surprisingly, samples from the surface soil of ancient copper mining areas supplied mostly strains with a moderate sensitivity to Cu2+, Ni2+, and Co2+, but in contrast, a soil sample from an excavation site of a medieval city that had been covered for about eight centuries showed an extremely high tolerance against cobalt ion (up to 36 mM). The differences among the strains not only may be regarded as results of adaptation to the different environmental conditions faced by the strains in nature but also seem to be related to ancient human activities and temporal partial decoupling of soil elements from the surface. This investigation confirmed that microfluidic screening offers empirical characterization of properties from same species which has been isolated from sites known to have different human activities in the past.
Strutynska, Nataliia; Malyshenko, Anna; Tverdokhleb, Nina; Evstigneev, Maxim; Vovchenko, Ludmila; Prylutskyy, Yuriy; Slobodyanik, Nikolai; Ritter, Uwe;
Design, characterization and mechanical properties of new Na+, CO32--apatite/alginate/C60 fullerene hybrid biocomposites. - In: Journal of the Korean Ceramic Society. - [Singapore] : Springer Singapore, ISSN 2234-0491, Bd. 58 (2021), 4, S. 422-429
- Im Titel sind "+" und "2-" hochgestellt, "3" und "60" tiefgestellt

Nanoparticles (20-50 nm) of Na+, CO32--containing calcium phosphate (Na: 1.49 wt% and C: 1.53 wt%) with apatite-type structure were prepared by precipitation method from aqueous solution. According to FTIR spectroscopy data, the partial substitution of phosphate by carbonate (B-type) realized in the apatite-type structure. Obtained Na+, CO32--hydroxyapatite (HAP) was used for the preparation of hybrid biocomposites with Alginate (Alg) with weight ratio HAP: Alg = 1:1 or 2:1 and C60 fullerene (C60; from 0.2 to 4 wt%) and their mechanical properties were determined. It was found, that sample with weight ratio HAP: Alg = 2:1 and containing 4.0 wt% of C60 has the highest Young's modulus 429 MPa comparing with other determined samples. The structure modeling of the investigated system showed that the formation of triple complexes Na+, CO32--HAP-Alg-C60 is stabilized by solvophobic and stacking interactions. The created biocomposites can be used as an effective implant material for bone restoration.